Fig. 9. Effect of VDAC1^-/- on Bax association with mitochondria. Western blot analyses of Bax levels in mitochondria (A) and cytosol (B) isolated from WT and VDAC1^-/-C1 H9c2 cells treated with or without 20 µM of ROT for 20 h. COX IV, GAPDH and β-tubulin were used as protein loading control and as mitochondrial and cytosol markers. *P<0.05 vs. WT non-ROT. ^#P<0.05 vs. VDAC1^-/-C1 non-ROT. Each experiment was repeated three times.